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1. Introduction

"Life analytical chemistry" is the study of various components, structural units and changes in the process of interaction in the life system

To establish new principles, methods, and technologies for biomolecular detection in the process of life activities, to conduct specific, sensitive, and rapid qualitative and quantitative monitoring of living substances, and to achieve molecular recognition and biological information extraction as an emerging interdisciplinary field.

In the past 20 years,life sciencesFlourishing development, therefore,Life analytical chemistry,Life analytical chemistry,life sciencesLife analytical chemistry.

1.1 Application of Microchip Electrophoresis Technology in Life Analysis
Due to the presence of reagents and samples in MCELow consumption, high separation efficiency, fast analysis speed, high detection sensitivity, and low analysis cost,Life analytical chemistry.MCE.

• Application of Microchip Electrophoresis Technology in Nucleic Acid Detection

DNARNA,.LiCombined MCE and CL technologies,microRNA.MCE-CL1-1.

,,miR-30b/Probe,G4DNA,G4DNA,Nb.BbvCI,DNA,.

Figure 1.1 High sensitivity detection analysis of microRNA based on MCE-CL new sensing platform

• Application of Microchip Electrophoresis Technology in Protein Detection

,.The channel size is very smallAt the same time, it also meansVery low sample consumption,.

,.QinUsing microfluidic chipsElectrophoresis driven controlled electroosmosis to form MCE-LIF platform using Laser-induced fluorescence,,,,,.

• Application of Microchip Electrophoresis Technology in Cell Detection

Rakszewska proposed a method based onDroplet microfluidic platformmRNA.

DNA functionalized hydrogel beads are used as a matrix to capture mRNA in single cells, and thenReverse transcription,cDNA,RCA,RCA,mRNA.,.

1.4Droplet microfluidic platformmRNA

2. Detection of p53 gene using microchip electrophoresis separation assisted chemiluminescence signal amplification

2.1 Method Design and Experimental Principles

The experimental principle is shown in Figure 2-1. The probe single stranded DNA (HRP-DNA) labeled with horseradish peroxidase (HRP) is partially complementary to the target p53,Hybridization of probe DNA with target p53,

T7Exo,T7Exo5'3'RNA/DNADNA,DNA,DNAp53HRP-DNA,T7Exo,,p53,Target signal amplification.

Due toHRP-DNA,,HRP,HRP-DNA,Catalytic oxidation of luminol by H2O2 to produce chemiluminescence.

MCE-CLHRP,HRP-DNA,Due to,It will generate two peaks, one in front and one in back,HRP,p53.

Figure 2-1 Experimental principle of detecting p53 using T7Exo assisted signal amplification MCE-CL

,Three samples with different conditionsMCE-CL,2-2,Blank testOnly appears whenOne electrophoresis.

,p53,HRP-DNA,Due top53T7ExoHRP-DNAHRP,

Due toThe charge to mass ratio of the two is different,,HRP,HRP-DNA.

Figure 2-2 Electrophoretic Diagram of Principle Feasibility Verification Experiment

(a)8.010-8mol/LHRP-DNA,(b)8.010-8mol/LHRP-DNA+1.010-10mol/Lp53,(c)8.010-8mol/LHRP-DNA+2.010-9mol/Lp53.1HRP-DNA2HRP.

2.2 Conclusion

This study established aMCE-CL analysis methodp53,.,60s.

p539.010-12mol/L2.010-8mol/L,concentrationCL,4.810-12mol/L.,,T7Exo,MCE-CL.

3. Detection of HIV-DNA using microchip electrophoresis separation assisted chemiluminescence signal amplification

3.1 Method Design and Experimental Principles

Based on microchip electrophoresis withOnline separation function,,.

MCE-CL,HRP-DNA,HRPDNA,T7Exo,MCE,HIV-DNAHigh sensitivity detection,3-1.

Figure 3-1 Experimental principle of enzymatic digestion assisted HIV-DNA double cycle signal amplification reaction

T7Exo-HRP-MCE-CL,Three samples with different conditionsMCE-CL,3-2.

When the sampleTarget not addedHIV-DNA,HRP-DNA,One electrophoresis.,HIV-DNA,HRP,HRPThe corresponding peak height also increases.

HRP,HRPHIV-DNA.

Figure 3-2 Electrophoresis diagram of feasibility validation test

(a)8.010-8mol/LHRP-DNA,(b)8.010-8mol/LHRP-DNA+1.010-12mol/LHIV-DNA,(c)8.010-8mol/LHRP-DNA+1.010-9mol/LHIV-DNA.1HRP-DNA2HRP

3.2 Conclusion

This study established a method based on the T7Exo HRP MCE-CL platformA New MCE Separation Assisted Double Cycle Chemiluminescence Signal Amplification Method,HIV-DNAHigh sensitivity detection.MCE-CL,60s.

,Double amplification of target signal,,1.610-15mol/LS/N=3,,,HIV-DNA,MCE-CL.

4. Chemiluminescence detection of S1 nuclease activity and its inhibitor based on microchip electrophoresis separation

4.1 Method Design and Experimental Principles

Based on microchip electrophoresis withOnline separation function,,.CL,.

4-1.,S1DNAHydrolysis cutting,S1S1neclease,S1necleaseHRP-ssDNAHRP.

,HRP,HRP-DNA,Catalytic oxidation of luminol by H2O2 to produce chemiluminescence.

Relevant literature reports that PPi has certain effect on S1 nuclease activityinhibition,S1DNAconcentrationPPi.MCE-CLPPiS1DNAinhibition.

,inhibitionPPiconcentration,.,PPiATP,S1DNA.

MCE-CLATPS1DNAinhibition.ATPS1FAM-ssDNAinhibition.

,MCE-CLS1,MCE-CLS1inhibitionPPiATP.

,Three samples with different conditionsMCE-CL,4-2,When the sampleTarget not addedS1,HRP-DNA,One electrophoresis.

,S1,HRP-DNA,Due toThe charge to mass ratio of the two is different,,HRP2,HRP-DNA1.

Figure 4-2 Feasibility Verification Electrophoresis Map

(a)8.010-8mol/LHRP-DNA,(b)8.010-8mol/LHRP-DNA+1.010-3U/mLS1,(c)8.010-8mol/LHRP-DNA+2.010-2U/mLS1.1HRP-DNA2HRP

4.2 Conclusion

This article utilizesDNA-HRP,S1,,Suitable for complex biological systems.

,,S1concentration1.010-1U/mL3.010-4U/mL,concentration,S/N=31.5610-4U/mL.

S1inhibitionATP,PPiinhibition,S1inhibition,.

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